abstract：:According to the Cholodny-Went hypothesis, gravitropic differential growth is brought about by the redistribution of auxin (indolyl-3-acetic acid, IAA). We reinvestigated the relevance of different auxins and studied the role of ethylene in hypocotyls of sunflower and shoots and roots of rye and maize seedlings. Incub...

journal_title：Protoplasma

authors： Edelmann HG,Roth U

更新日期：2006-12-01 00:00:00

abstract：:The effects of the calcium sequester EGTA on gravitactic orientation and membrane potential changes in the unicellular flagellate Euglena gracilis were investigated during a recent parabolic-flight experiment aboard of an Airbus A300. In the course of a flight parabola, an acceleration profile is achieved which yields...

journal_title：Protoplasma

authors： Richter PR,Schuster M,Meyer I,Lebert M,Häder DP

更新日期：2006-12-01 00:00:00

abstract：:We investigated the diurnal differences in the innermost surface of tracheid cell walls at various developmental stages from cambium to mature xylem. Cryptomeria japonica saplings were cultivated in a growth chamber with a light cycle set at 14 h of light and 10 h of darkness. Samples were collected from the saplings ...

journal_title：Protoplasma

authors： Hosoo Y,Imai T,Yoshida M

更新日期：2006-11-01 00:00:00

abstract：:Fibrillarin and the plant nucleolin homolog NopA64 are two important nucleolar proteins involved in pre-rRNA processing. In order to determine the effects of the altered gravity environment on the nucleolus, we have investigated the location of fibrillarin and NopA64 in nucleolar subcomponents of cress (Lepidium sativ...

journal_title：Protoplasma

authors： Sobol M,Gonzalez-Camacho F,Rodríguez-Vilariño V,Kordyum E,Medina FJ

更新日期：2006-09-01 00:00:00

abstract：:Various stresses such as starvation and cold or heat shocks have been identified as triggers in the induction of the microspore embryogenesis. This study attempts to quantify the effects of different pretreatment conditions for successful microspore culture of malting barley (cv. Scarlett). While the sporophytic micro...

journal_title：Protoplasma

authors： Oleszczuk S,Sowa S,Zimny J

更新日期：2006-08-01 00:00:00

abstract：:Two novel beta-expansin genes, TaEXPB1 and TaEXPB2, were isolated from wheat microspores by suppression subtractive hybridisation. Northern blot and reverse transcription PCR analyses showed that the expression of both genes was restricted to early stages of male gametophyte development (from microspores to immature p...

journal_title：Protoplasma

authors： Jin Y,Tashpulatov AS,Katholnigg H,Heberle-Bors E,Touraev A

更新日期：2006-08-01 00:00:00

abstract：:Transmission electron microscopy of immunogold-labeled Chinese pine egg cells before and after fertilization revealed that polysomes are associated with microtubules (MTs) from fertilization to the 2-nucleate embryo stage. Ribosome aggregates of various size and shape were randomly distributed in the cytoplasm of the ...

journal_title：Protoplasma

authors： Han Y,Yu J,Guo F,Watkins SC

更新日期：2006-05-01 00:00:00

abstract：:The mature apomictic embryo sac of Chondrilla juncea is highly vacuolated and demonstrates a polarization similar to that of the amphimictic gametophyte. The microtubule cytoskeleton of this embryo sac is uncharacteristic and relatively weak. The microtubules are positioned along cell walls and resemble cortical micro...

journal_title：Protoplasma

authors： Kościńska-Pajak M,Bednara J

更新日期：2006-05-01 00:00:00

abstract：:Previous complementation of a chitin synthase class Vb null mutant (Colletotrichum graminicola chsA) indicated that the encoded protein is responsible for approximately 30% of the conidial chitin, is essential for conidial wall strength in media with high water potential, and contributes to strength of hyphal tips. We...

journal_title：Protoplasma

authors： Amnuaykanjanasin A,Epstein L

更新日期：2006-05-01 00:00:00

abstract：:This paper reports an efficient protoplast culture technique, the "extra thin alginate film" technique. The development of this improved method of protoplast culture was an outcome of an assessment of the efficiency and shortcomings of various protoplast culture techniques. The efficiency of this technique was evaluat...

journal_title：Protoplasma

authors： Pati PK,Sharma M,Ahuja PS

更新日期：2005-12-01 00:00:00

abstract：:We used a noninvasive microprobe technique to record in substomatal cavities of barley leaves the apoplastic pH response to different stress situations. When K+ (or Na+) activity at the roots of intact plants was increased from 1 to 50 mM, the leaf apoplastic pH increased by 0.4 to 0.6 units within 8 to 12 min when st...

journal_title：Protoplasma

authors： Felle HH,Herrmann A,Hückelhoven R,Kogel KH

更新日期：2005-12-01 00:00:00

abstract：:The aim of the study was to elucidate the effect of cadmium ions on the arrangement of the actin and tubulin cytoskeleton, as well as the relationships between cytoskeletal changes and growth processes in the green filamentous alga Spirogyra decimina. Batch cultures of algae were carried out under defined conditions i...

journal_title：Protoplasma

authors： Pribyl P,Cepák V,Zachleder V

更新日期：2005-12-01 00:00:00

abstract：:Plant development is regulated by numerous chemicals derived from a multitude of metabolic pathways. However, we know very little about the biological effects and functions of many of these metabolites in the cell. N-Acylethanolamines (NAEs) are a group of lipid mediators that play important roles in mammalian physiol...

journal_title：Protoplasma

authors： Motes CM,Pechter P,Yoo CM,Wang YS,Chapman KD,Blancaflor EB

更新日期：2005-12-01 00:00:00

abstract：:The directional elongation of root hairs, "tip growth", depends on the coordinated and highly regulated trafficking of vesicles which fill the tip cytoplasm and are active in secretion of cell wall material. So far, little is known about the dynamics of endocytosis in living root hairs. We analyzed the motile behaviou...

journal_title：Protoplasma

authors： Ovecka M,Lang I,Baluska F,Ismail A,Illes P,Lichtscheidl IK

更新日期：2005-10-01 00:00:00

abstract：:We used qualitative and quantitative fluorescence microscopy of the fluorescein isothiocyanate-conjugated lectins Concanavalin A, phytohaemagglutinin-erythroagglutinin, pokeweed mitogen, and peanut agglutinin to examine sugar composition on the cell surface and cell-associated mucilage (where present) in a number of c...

journal_title：Protoplasma

authors： Tien CJ,Sigee DC,White KN

更新日期：2005-10-01 00:00:00

abstract：:Microtubule dynamics were examined in live cells of the fungal plant pathogen Magnaporthe grisea transformed for constitutive expression of a fusion protein containing enhanced yellow-fluorescent protein and a Neurospora crassa benomyl-resistant allele of beta-tubulin. Transformants retained their ability to different...

journal_title：Protoplasma

authors： Czymmek KJ,Bourett TM,Shao Y,DeZwaan TM,Sweigard JA,Howard RJ

更新日期：2005-04-01 00:00:00

abstract：:The extent to which the actomyosin motor responsible for cytoplasmic streaming contributes to the translational diffusion of water in Elodea canadensis cells was studied by a nuclear magnetic resonance (NMR) spin-echo technique. The relative contribution of the actomyosin motor was determined from the corresponding ap...

journal_title：Protoplasma

authors： Vorob'ev VN,Anisimov AV,Dautova NR

更新日期：2004-12-01 00:00:00

abstract：:Programmed cell death occurs in plants during several developmental processes and during the expression of resistance to pathogen attack (i.e., the hypersensitive response). An unsolved question of plant programmed cell death is whether a unique signaling pathway or different, possibly convergent pathways exist. This ...

journal_title：Protoplasma

authors： Malerba M,Cerana R,Crosti P

更新日期：2004-10-01 00:00:00

abstract：:In the present study energy-filtering transmission electron microscopy by use of an in-column spectrometer is employed as a powerful tool for ultrastructural analysis of plant cells. Images of unstained very thin (50 nm) and thick (140 nm) sections of the unicellular green alga Micrasterias denticulata, as a model sys...

journal_title：Protoplasma

authors： Lütz-Meindl U,Aichinger N

更新日期：2004-06-01 00:00:00

abstract：:Along the past recent years, two-photon excitation (TPE) microscopy has moved from the realms of technical curiosity to be a standard application in many advanced cell biology laboratories. The growing body of literature covered in this review points out the obvious advantages of TPE over any other imaging method base...

journal_title：Protoplasma

authors： Feijó JA,Moreno N

更新日期：2004-03-01 00:00:00

abstract：:The actin-nucleating and -organizing Arp2/3 protein complex is well known to be conserved throughout the eukaryotic kingdom. For higher plants, however, only limited evidence is available for the presence of the Arp2/3 complex so far. Using heterologous antibodies against the Dictyostelium discoideum and Schizosacchar...

journal_title：Protoplasma

authors： Van Gestel K,Slegers H,Von Witsch M,Samaj J,Baluska F,Verbelen JP

更新日期：2003-09-01 00:00:00

abstract：:Some species of Spirogyra form rosette-shaped or rod-shaped rhizoids in the terminal cell of the filaments. In the present study, we analyzed an involvement of microtubules (MTs) in rhizoid differentiation. Before rhizoid differentiation, cortical MTs were arranged transversely to the long axis of cylindrical cells, r...

journal_title：Protoplasma

authors： Yoshida K,Inoue N,Sonobe S,Shimmen T

更新日期：2003-06-01 00:00:00

abstract：:The aim of this work was to study the activity of NAD(P)H:(quinone acceptor) oxidoreductase 1 (EC 1.6.99.2) in the regeneration of lipophilic antioxidants, alpha-tocopherol, and reduced-coenzyme Q analogs. First, we tested whether or not two isoforms of the NAD(P)H:(quinone acceptor) oxidoreductase 1 designated as "hy...

journal_title：Protoplasma

authors： Bello RI,Kagan VE,Tyurin V,Navarro F,Alcaín FJ,Villalba JM

更新日期：2003-05-01 00:00:00

abstract：:We examined the nature of the posttranslational modification of bovine cytochrome b(561), a membrane-spanning protein and an essential component of neuroendocrine secretory vesicles. Matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF-MS) showed two populations in the partially ...

journal_title：Protoplasma

authors： Nakamura M,Takeuchi F,Tsubaki M

更新日期：2003-05-01 00:00:00

abstract：:While there is a number of studies on the effects of Rho GTPases on the actin-based cytoskeleton in higher eukaryotes, studies in protozoans are rather limited. The problem seems to be intriguing since the structure of protozoan cytoskeletons is distinct from most vertebrate cells. By blocking endogenous Rho family pr...

journal_title：Protoplasma

authors： Kłopocka W,Redowicz MJ

更新日期：2003-03-01 00:00:00

abstract：:It is well established that cytoplasmic microtubules are depolymerized during nuclear division and reassembled as mitotic microtubules. Mounting evidence showing that cytoplasmic microtubules were also involved in apical growth of fungal hyphae posed the question of whether apical growth became disrupted during nuclea...

journal_title：Protoplasma

authors： Riquelme M,Fischer R,Bartnicki-García S

更新日期：2003-01-01 00:00:00

abstract：:The ultrastructure of the several micrometers thick cytoplasmic layer of the giant marine alga Valonia utricularis displays characteristics which are apparently linked with the capability of this alga to regulate turgor pressure. Transmission and scanning electron microscopy of cells prefixed in different ways, includ...

journal_title：Protoplasma

authors： Mimietz S,Heidecker M,Krohne G,Wegner LH,Zimmermann U

更新日期：2003-01-01 00:00:00

abstract：:Syagrus coronata is an economically important palm tree grown as an ornament, for the oil extracted from its seeds, and the wax from its leaves which has several applications in industry. Silicon biocomposites were analyzed in leaves of S. coronata. Silica bodies were found as extracellular silica masses between the h...

journal_title：Protoplasma

authors： Lins U,Barros CF,da Cunha M,Miguens FC

更新日期：2002-10-01 00:00:00

abstract：:A survey is presented of the architecture of secondary wall ingrowths in transfer cells from various taxa based on scanning electron microscopy. Wall ingrowths are a distinguishing feature of transfer cells and serve to amplify the plasma membrane surface area available for solute transport. Morphologically, two categ...

journal_title：Protoplasma

authors： Talbot MJ,Offler CE,McCurdy DW

更新日期：2002-05-01 00:00:00

abstract：:Dehydroleucodine is a sesquiterpene lactone recently isolated from aerial parts of a medicinal herb, Artemisia douglasiana Besser. We have previously shown that 25 and 100 microM dehydroleucodine slowed down onion root growth by 30 and 70%, respectively, affecting neither cell viability nor cell elongation. In the pre...

journal_title：Protoplasma

authors： López ME,Giordano OS,López LA

更新日期：2002-02-01 00:00:00

abstract：:The LIM domain is an evolutionary conserved double-zinc finger motif found in a variety of proteins exhibiting diverse biological roles. LIM domains have been observed to act as modular protein-binding interfaces mediating protein-protein interactions in the cytoplasm and the nucleus. Interaction of LIM domains with s...

journal_title：Protoplasma

authors： Khurana T,Khurana B,Noegel AA

更新日期：2002-02-01 00:00:00

abstract：:The detergent Brij 58 has been introduced to reverse plasma membrane (PM) vesicles from the right-side-out to the inside-out form. The aim of the present work was to investigate the effect of Brij 58 on the formation of an ATP-dependent proton gradient and on the fluidity of the lipid phase of PM vesicles. PMs of corn...

journal_title：Protoplasma

authors： Behzadipour M,Kluge M,Lüthje S

更新日期：2001-01-01 00:00:00

abstract：:Ascorbate has been related to the differentiation of several mesenchymal cells including haematopoietic cells. We have previously demonstrated that ascorbate enhances the activity of 1 alpha,25-dihydroxyvitamin D3 (1 alpha,25(OH)2D3) on monocytic differentiation of HL-60 cells. Here, we show that ascorbate-mediated mo...

journal_title：Protoplasma

authors： López-Lluch G,Blázquez MV,Pérez-Vicente R,Macho A,Burón MI,Alcaín FJ,Muñoz E,Navas P

更新日期：2001-01-01 00:00:00

abstract：:Heat shock protein 90 (Hsp90) is an abundant cytosolic protein. In higher eukaryotes two isoforms of Hsp90 exist, Hsp90 alpha and Hsp90 beta. Hsp90 was purified from rat liver and after sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a double band at about 90 kDa. The two bands were separated and id...

journal_title：Protoplasma

authors： Langer T,Fasold H

更新日期：2001-01-01 00:00:00

abstract：:19F nuclear magnetic resonance (NMR) imaging and 19F NMR chemical-shift imaging (19F CSI) have been used to localize fluorinated compounds administered to stems of Ancistrocladus heyneanus and A. abbreviatus for the elucidation of biosynthetic pathways in living plants. This first application of 19F CSI on plants prov...

journal_title：Protoplasma

authors： Bringmann G,Wolf K,Meininger M,Rokitta M,Haase A

更新日期：2001-01-01 00:00:00

abstract：:Fluorescence in situ hybridization revealed the presence of ribosomal RNA genes in paracentromeric regions of all A chromosomes and in the distal half of B chromosomes in embryonic cells from Moroccan specimens of the grasshopper Eyprepocnemis plorans. The expression of these genes was monitored by the presence of nuc...

journal_title：Protoplasma

authors： Bakkali M,Cabrero J,López-León MD,Perfectti F,Camacho JP

更新日期：2001-01-01 00:00:00

abstract：:The dinoflagellate Crypthecodinium cohnii Biecheler propagates by both binary and multiple fission. By a newly developed mutagenesis protocol based on using ethyl methanesulfonate and a cell size screening method, a cell cycle mutant, mf2, was isolated with giant cells which predominantly divide by multiple fission. T...

journal_title：Protoplasma

authors： Lam CM,Chong C,Wong JT

更新日期：2001-01-01 00:00:00

abstract：:Mechanisms of transport of secretory products across the Golgi apparatus (GA) as well as of scale formation in prymnesiophytes have remained controversial. We have used a quantitative morphological approach to study formation and transport of scales across the GA in haploid cells of Pleurochrysis sp. The GA of these c...

journal_title：Protoplasma

authors： Hawkins EK,Lee JJ

更新日期：2001-01-01 00:00:00

abstract：:Tradescantia virginiana leaf epidermal cells were plasmolysed by sequential treatment with 0.8 M and 0.3 M sucrose. Plasmolysis revealed adhesion of the plasma membrane to the cell wall at sites coinciding with cytoskeletal arrays involved in the polarisation of cells undergoing asymmetric divisions--cortical actin pa...

journal_title：Protoplasma

authors： Cleary AL

更新日期：2001-01-01 00:00:00

abstract：:Through reverse transcription-polymerase chain reaction and Northern blot analysis, we identified TBK5, a novel plant-specific kinesin-like protein (KLP) that is highly expressed in interphase tobacco BY-2 cells. TBK5 mRNA was present at a high level throughout the growth cycle, even in cells that had entered the stat...

journal_title：Protoplasma

authors： Matsui K,Collings D,Asada T

更新日期：2001-01-01 00:00:00